Cerebellar afferents from neurons in the extraocular motor nuclei: A fluorescent retrograde double-labeling study in the sheep

Gold labeling of two antigens (double labeling) is often done on two section surfaces separated by section thickness. Whether labeling is done on both sides of the same section or on two parallel surfaces separated by section thickness (PSSST), comparable results are dependent on an equal number of epitopes being exposed at each surface. We propose a method to study protein labeling within the same field of proteins, by examining two directly adjacent surfaces that were split during sectioning. The number of labeling sites on adjacent surfaces (AS) were compared to sites on PSSST surfaces in individual bacteria.Since each bacteria needed to be recognizable in all three section surfaces, one-hole grids were used for labeling. One-hole grids require a supporting membrane and excessive handling during labeling often ruptures the membrane. To minimize handling, a labeling chamber was designed that is inexpensive, disposable, minimizes contamination, and uses a minimal amount of solution.

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Faculty Opinions recommendation of Short-term synchrony in diverse motor nuclei presumed to receive different extents of direct cortical input.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.717960176.793463358 ◽

2012 ◽

Author(s):

William Z Rymer ◽

Renee Theiss

Keyword(s):

Short Term ◽

Cortical Input ◽

Motor Nuclei ◽

To Receive

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Determination of glycolic acid level in higher plants during photorespiration by stable isotope dilution mass spectrometry with double-labeling experiments

Analytical Biochemistry ◽

10.1016/0003-2697(85)90012-0 ◽

1985 ◽

Vol 147 (1) ◽

pp. 86-91 ◽

Cited By ~ 5

Author(s):

Pascale Jolivet ◽

Pierre Gans ◽

Christian Triantaphylides

Keyword(s):

Mass Spectrometry ◽

Stable Isotope ◽

Isotope Dilution ◽

Acid Level ◽

Glycolic Acid ◽

Higher Plants ◽

Isotope Dilution Mass Spectrometry ◽

Double Labeling ◽

Stable Isotope Dilution

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Innervation of Epi- and Endoneurial Compartments of Rat Facial, Vagus and Sciatic Nerves as Studied by Double-Labeling Immunofluorescence

Cells Tissues Organs ◽

10.1159/000147587 ◽

1994 ◽

Vol 149 (4) ◽

pp. 264-271 ◽

Cited By ~ 5

Author(s):

W. Kummer ◽

E. Seifert ◽

A. Schadel

Keyword(s):

Double Labeling ◽

Sciatic Nerves

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Expression and distribution of the Na+- HCO 3 − cotransporter in human pancreas

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.1999.277.2.g487 ◽

1999 ◽

Vol 277 (2) ◽

pp. G487-G494 ◽

Cited By ~ 32

Author(s):

Christopher R. Marino ◽

Virginia Jeanes ◽

Walter F. Boron ◽

Bernhard M. Schmitt

Keyword(s):

Northern Blot ◽

Islet Cells ◽

Rat Kidney ◽

Physiological Role ◽

Human Pancreas ◽

Transmembrane Conductance Regulator ◽

Transmembrane Conductance ◽

Cellular Mechanisms ◽

Double Labeling ◽

Normal Human

The cellular mechanisms of [Formula: see text] secretion in the human pancreas are unclear. Expression of a Na+-[Formula: see text]cotransporter (NBC) mRNA has been observed recently, but the distribution and physiological role of the NBC protein are not known. Here we examined the expression and localization of NBC in human pancreas by Northern blot, immunoblot, and immunofluorescence microscopy. Rat kidney NBC probes detected a single 9.5-kb band by Northern blot. On immunoblots, two polyclonal antisera directed against different epitopes of rat kidney NBC identified a single ∼130-kDa protein. In cryosections of normal human pancreas, both antisera labeled basolateral membranes of large, morphologically identifiable ducts and produced a distinct labeling pattern in the remainder of the parenchyma. In double-labeling experiments, NBC immunoreactivity in the parenchyma colocalized with the Na+-K+pump, a basolateral marker. In contrast, NBC and cystic fibrosis transmembrane conductance regulator, an apical membrane marker, were detected within the same histological structures but at different subcellular localizations. The NBC antisera did not label acinar or islet cells. Our observations suggest that secretion of[Formula: see text] by human pancreatic duct cells involves the basolateral uptake of Na+and[Formula: see text] via NBC, an electrogenic Na+-[Formula: see text]cotransporter.

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